DNA Damage Assay
Various methods are utilized to impose damage to the genome of a cell. The DNA damage response (DDR) varies depending on the method of DNA damage induction. Below are some common methods of inducing DNA damage in cell culture-based assays:
1) Cisplatin (in the form of CDDP) - a chemotherapeutic which passively diffuses into cells and forms adducts with DNA that can be detected using the IC4 antibody to verify delivery of the drug in the cells.
2) Ionizing radiation (IR) - causes both single- and double-stand breaks in DNA
3) Etoposide - inhibits topoisomerase II activity and results in predominantly double-strand breaks in late S-phase
4) Mitomycin C - an alkylating agent which causes DNA cross-links and double strand breaks, similar to the effects of CDDP.
5)
1) Cisplatin (in the form of CDDP) - a chemotherapeutic which passively diffuses into cells and forms adducts with DNA that can be detected using the IC4 antibody to verify delivery of the drug in the cells.
2) Ionizing radiation (IR) - causes both single- and double-stand breaks in DNA
3) Etoposide - inhibits topoisomerase II activity and results in predominantly double-strand breaks in late S-phase
4) Mitomycin C - an alkylating agent which causes DNA cross-links and double strand breaks, similar to the effects of CDDP.
5)