Illumina HiSeq
Sequencing by synthesis
Multiplexed sequencing
In the multiplexed sequencing method, DNA libraries are “tagged” with a unique identifier, or index, during sample preparation. Multiple samples are then pooled into a single lane on a flow cell and sequenced together in one Genome Analyzer run.
- DNA is isolated and purified (ex. ChIP DNA)
- 'Library prep' follows to prepare DNA for sequencing
- DNA is end repaired (via transposomes)
- DNA is ligated with flanking adapters containing: a unique index sequence, sequencing primer binding sites, and regions that are complimentary to the flow cell oligo.
- Clustering -> Flow cell
Multiplexed sequencing
In the multiplexed sequencing method, DNA libraries are “tagged” with a unique identifier, or index, during sample preparation. Multiple samples are then pooled into a single lane on a flow cell and sequenced together in one Genome Analyzer run.