Gene targeting
Achieving ubiquitous expression (whole body)
ROSA26 locus
ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice.[1] It was first isolated in 1991[2] in a gene-trap mutagenesis screen ofembryonic stem cells (ESCs). Over 130 knock-in lines have been created based on the ROSA26 locus.[3] The human homolog of the ROSA26 locus has been identified
Targeting vs. transgenic
ROSA26 locus
ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice.[1] It was first isolated in 1991[2] in a gene-trap mutagenesis screen ofembryonic stem cells (ESCs). Over 130 knock-in lines have been created based on the ROSA26 locus.[3] The human homolog of the ROSA26 locus has been identified
Targeting vs. transgenic